Deletion analysis of the c‐Ha‐ras oncogene promoter

FEBS Letters

Trimble, William S.²  Hozumi, Nobumichi¹ 

[1] Departments of Medical Genetics, University of Toronto, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada;Division of Immunology and Neurobiology, Mount Sinai Hospital Research Institute, University of Toronto, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada

关键词: c-Ha-ras oncogene;    Promoter;    Deletion analysis;    Chloramphenicol acetyltransferase;    Enzyme assay;    Consensus box;    (Human);   

DOI  :  10.1016/0014-5793(87)81192-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using transient assays of chloramphenicol acetyltransferase expression in CV-1 cells, we have localized the human c-Ha-ras oncogene promoter to a 550 bp fragment located about 1 kb upstream from the ras coding sequence. Deletion analysis has revealed that a 100 nucleotide region 200 base pairs upstream of the putative initiation sites for transcription is essential for high levels of expression. Within this sequence lie two Spl binding sites and a consensus CCAAT box.

【 授权许可】

Unknown