Enterococcus hirae vacuolar ATPase is expressed in response to pH as well as sodium

FEBS Letters

Igarashi, Kazuei¹  Takase, Kazuma²  Yamato, Ichiro²  Ikegami, Miho¹  Kakinuma, Yoshimi¹  Kawano, Miyuki¹ 

[1] Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan;Department of Biological Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, Chiba 278-8510, Japan

关键词: Vacuolar ATPase;    ntp operon;    Promoter;    Chloramphenicol acetyltransferase;    Enterococcus hirae;   

DOI  :  10.1016/S0014-5793(99)00776-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Enterococcus hirae ntp operon encodes both a vacuolar ATPase, which transports Na⁺ as well as Li⁺, and the KtrII K⁺ transporter. A plasmid, in which the chloramphenicol acetyltransferase gene (CAT) was placed downstream of the ntp promoter, was introduced into a mutant totally defective in Na⁺ extrusion. The CAT activity of this transformant was increased preferentially by addition of NaCl, but not by LiCl, in the media or by elevating the medium pH, correlating well with the increase in amounts of the ATPase subunits observed by Western blotting. The physiological significance of these responses of the ntp promoter is discussed.

【 授权许可】

Unknown