Prevalence and molecular characterization of Mycobacterium tuberculosis complex in cattle and humans, Maiduguri, Borno state, Nigeria: a cross-sectional study

BMC Microbiology

article

Kwaghe, Ayi Vandi¹  Ameh, James Agbo³  Kudi, Caleb Ayuba⁴  Ambali, Abdul-Ganiyu⁵  Adesokan, Hezekiah Kehinde⁶  Akinseye, Victor Oluwatoyin⁶  Adelakun, Olubukola Deborah⁶  Usman, Joy Gararawa⁸  Cadmus, Simeon Idowu⁶ 

[1] Department of Veterinary and Pest Control Services, Federal Ministry of Agriculture and Rural Development;Nigeria Field Epidemiology and Laboratory Training Programme;Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Abuja;Department of Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria;Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ilorin;Department of Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ibadan;Department of Chemical Sciences, Augustine University Ilara-Epe;National Veterinary Research Institute

关键词: Prevalence;    Bovine and human tuberculosis;    Culture and isolation;    Genus typing;    Deletion analysis;    Spoligotyping;    Mycobacterium tuberculosis complex;   

DOI  :  10.1186/s12866-022-02710-y

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Globally, the highest burden of bovine and human tuberculosis resides in Africa and Asia. Tuberculosis (TB) is the second leading single infectious killer after severe acute respiratory syndrome corona virus-2 (SARSCOV-2). Bovine TB remains a treat to wild and domesticated animals, humans and hinders international trade in endemic countries like Nigeria. We aimed at determining the prevalence of bovine and human tuberculosis, and the spoligotypes of Mycobacterium tuberculosis complex in cattle and humans in Maiduguri. We conducted a cross sectional study on bovine and human tuberculosis in Maiduguri, Borno state. We calculated sample size using the method of Thrusfield. Lesions suggestive of TB from 160 slaughtered cattle were obtained from Maiduguri Central Abattoir. Sputum samples from humans; 82 abattoir workers and 147 suspected TB patients from hospitals/clinics were obtained. Lesions and sputum samples were cultured for the isolation of Mycobacterium spp. Positive cultures were subjected genus typing, deletion analysis and selected isolates were spoligotyped. Data was analysed using SPSS VERSION 16.0. Prevalence of 32.5% (52/160) was obtained in cattle. Damboa local government area (LGA), where majority of the infected animals were obtained from had 35.5% bTB prevalence. All categories analysed (breed, age, sex, body conformation and score) had P-values that were not significant (P > 0.05). Sputum culture revealed a prevalence of 3.7% (3/82) from abattoir workers and 12.2% from hospitals/clinics. A significant P-value (0.03) was obtained when positive culture from abattoir and that of hospitals/clinics were compared. Out of the 52 culture positive isolates obtained from cattle, 26 (50%) belonged to M. tuberculosis complex (MTC) and 17/26 (65.4%) were characterized as M. bovis. In humans, 7/12 (58.3%) MTC obtained were characterized as M. tuberculosis. Spoligotyping revealed SB0944 and SB1025 in cattle, while SIT838, SIT61 of LAM10_CAM and SIT1054, SIT46 of Haarlem (H) families were obtained from humans. Cattle in Damboa LGA need to be screened for bTB as majority of the infected animals were brought from there. Our findings revealed the presence of SB0944 and SB1025 spoligotypes from cattle in Borno state. We isolated M. tuberculosis strain of the H family mainly domiciled in Europe from humans.

【 授权许可】

CC BY|CC0