Inhibition of DNA synthesis by phorbol esters through protein kinase C in cultured rabbit aortic smooth muscle cells

FEBS Letters

Yamamoto, Takeshi¹  Fukumoto, Yasuo¹  Takai, Yoshimi¹  Kariya, Ken-ichi²  Kawahara, Yasuhiro²  Fukuzaki, Hisashi²  Tsuda, Terutaka² 

[1] Departments of Biochemistry Kobe University School of Medicine, Kobe 650, Japan;Internal Medicine (1st Division), Kobe University School of Medicine, Kobe 650, Japan

关键词: Cell proliferation;    Phorbol ester;    Protein kinase C;    (Vascular smooth muscle);    SMCs;    smooth muscle cells;    PDGF;    platelet-derived growth factor;    EGF;    epidermal growth factor;    protein kinase A;    cyclic AMP-dependent protein kinase;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    PDBu;    phorbol-12;    13-dibutyrate;    4α-PDD;    4α-phorbol-12;    13-didecanoate;    Bt2cAMP;    dibutyryl cyclic AMP;    FCS;    fetal calf serum;    PDS;    plasma-derived serum;    WBS;    whole blood serum;    DMEM;    Dulbecco's modified Eagle's medium;   

DOI  :  10.1016/0014-5793(87)81245-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cultured rabbit aortic smooth muscle cells (SMC), 12-O-tetradecanoylphorbol-13-acetate (TPA) induced DNA synthesis in the presence of plasma-derived serum to a small extent, but inhibited markedly the rabbit whole blood serum (WBS)-, platelet-derived growth factor (PDGF)- and epidermal growth factor-induced DNA synthesis. Phorbol-12,13-dibutyrate (PDBu) mimicked this antiproliferative action of TPA, but 4α-phorbol-12, 13-didecanoate was inactive in this capacity. Prolonged treatment of the cells with PDBu caused the partial down-regulation of protein kinase C. In these protein kinase C-reduced cells, WBS still induced DNA synthesis, but TPA did not inhibit the WBS-induced DNA synthesis. We have previously shown that protein kinase C is involved at least partially in the PDGF-induced DNA synthesis in rabbit aortic SMC. The present results together with this earlier observation suggest that protein kinase C has not only a proliferative but also an antiproliferative action in rabbit aortic SMC.

【 授权许可】

Unknown