Distinct functions of down‐regulation‐sensitive and ‐resistant types of protein kinase C in rabbit aortic smooth muscle cells

FEBS Letters

Takai, Yoshimi¹  Kariya, Ken-ichi¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: Cell proliferation;    Ca2+;    Phorbol ester;    Protein kinase C;    (Vascular smooth muscle);    SMC;    smooth muscle cell;    WBS;    whole blood serum;    [Ca2+]i;    cytoplasmic free Ca2+ concentration;    PDBu;    phorbol-12;    13-dibutyrate;    FCS;    fetal calf serum;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    4α-PDD;    4α-phorbol-12;    13-didecanoate;    PDS;    plasma-derived serum;    DMEM;    Dulbecco's modified Eagle's medium;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(87)81202-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In quiescent cultures of rabbit aortic smooth muscle cells, 12-O-tetradecanoylphorbol-13-acetate (TPA) induced DNA synthesis to some extent in the presence of rabbit plasma-derived serum but inhibited the rabbit whole blood serum (WBS)-induced DNA synthesis and increase in cytoplasmic free Ca²⁺ concentration ([Ca²⁺]_i). Prolonged treatment of the cells with phorbol-12,13-dibutyrate (PDBu) caused the partial down-regulation of protein kinase C to a level of 25–35% of that in control cells. In these PDBu-pretreated cells, TPA neither induced DNA synthesis nor inhibited the WBS-induced DNA synthesis, but still inhibited the WBS-induced increase in [Ca²⁺]_i. These results suggest (i) that there are down-regulation-sensitive and -resistant types of protein kinase C in rabbit aortic smooth muscle cells; (ii) that the down-regulation-sensitive type has the proliferative and antiproliferative actions whereas the down-regulation-resistant type lacks them; and (iii) that the down-regulation-resistant type has the activity to inhibit the WBS-induced increase in [Ca²⁺]_i.

【 授权许可】

Unknown