FEBS Letters | |
Distinct functions of down‐regulation‐sensitive and ‐resistant types of protein kinase C in rabbit aortic smooth muscle cells | |
Takai, Yoshimi1  Kariya, Ken-ichi1  | |
[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan | |
关键词: Cell proliferation; Ca2+; Phorbol ester; Protein kinase C; (Vascular smooth muscle); SMC; smooth muscle cell; WBS; whole blood serum; [Ca2+]i; cytoplasmic free Ca2+ concentration; PDBu; phorbol-12; 13-dibutyrate; FCS; fetal calf serum; TPA; 12-O-tetradecanoylphorbol-13-acetate; 4α-PDD; 4α-phorbol-12; 13-didecanoate; PDS; plasma-derived serum; DMEM; Dulbecco's modified Eagle's medium; BSA; bovine serum albumin; | |
DOI : 10.1016/0014-5793(87)81202-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In quiescent cultures of rabbit aortic smooth muscle cells, 12-O-tetradecanoylphorbol-13-acetate (TPA) induced DNA synthesis to some extent in the presence of rabbit plasma-derived serum but inhibited the rabbit whole blood serum (WBS)-induced DNA synthesis and increase in cytoplasmic free Ca2+ concentration ([Ca2+]i). Prolonged treatment of the cells with phorbol-12,13-dibutyrate (PDBu) caused the partial down-regulation of protein kinase C to a level of 25–35% of that in control cells. In these PDBu-pretreated cells, TPA neither induced DNA synthesis nor inhibited the WBS-induced DNA synthesis, but still inhibited the WBS-induced increase in [Ca2+]i. These results suggest (i) that there are down-regulation-sensitive and -resistant types of protein kinase C in rabbit aortic smooth muscle cells; (ii) that the down-regulation-sensitive type has the proliferative and antiproliferative actions whereas the down-regulation-resistant type lacks them; and (iii) that the down-regulation-resistant type has the activity to inhibit the WBS-induced increase in [Ca2+]i.
【 授权许可】
Unknown
【 预 览 】
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