Luteinizing hormone‐releasing hormone (LHRH)‐induced arachidonic acid release in rat granulosa cells Role of calcium and protein kinase C

FEBS Letters

Minegishi, T.¹  Leung, P.C.K.¹  Wang, J.¹ 

[1] Departments of Obstetrics/Gynaecology and Physiology, University of British Columbia, Grace Hospital, Vancouver V6H 3 V5, Canada

关键词: Luteinizing hormone-releasing hormone;    Arachidonic acid;    Phorbol ester;    Ca2+;    Protein kinase C;    (Ovary);   

DOI  :  10.1016/0014-5793(87)80029-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat granulosa cells prelabeled with [³H]arachidonic acid, addition of LHRH, A23187 or 12-0-tetradecanoyl phorbol-13-acetate (TPA) enhanced the release of [³H]arachidonic acid into the culture medium. The effect of A23187 was significant as early as 5 min and the lowest effective dose was 5 × 10⁻⁸/M. On the other hand, TPA was effective only at dosages greater than 10⁻⁶M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca²⁺-dependent rather than a protein kinase C-mediated pathway.

【 授权许可】

Unknown