期刊论文详细信息
FEBS Letters | |
Luteinizing hormone‐releasing hormone (LHRH)‐induced arachidonic acid release in rat granulosa cells Role of calcium and protein kinase C | |
Minegishi, T.1  Leung, P.C.K.1  Wang, J.1  | |
[1] Departments of Obstetrics/Gynaecology and Physiology, University of British Columbia, Grace Hospital, Vancouver V6H 3 V5, Canada | |
关键词: Luteinizing hormone-releasing hormone; Arachidonic acid; Phorbol ester; Ca2+; Protein kinase C; (Ovary); | |
DOI : 10.1016/0014-5793(87)80029-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In rat granulosa cells prelabeled with [3H]arachidonic acid, addition of LHRH, A23187 or 12-0-tetradecanoyl phorbol-13-acetate (TPA) enhanced the release of [3H]arachidonic acid into the culture medium. The effect of A23187 was significant as early as 5 min and the lowest effective dose was 5 × 10−8/M. On the other hand, TPA was effective only at dosages greater than 10−6M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca2+-dependent rather than a protein kinase C-mediated pathway.
【 授权许可】
Unknown
【 预 览 】
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RO201912020289073ZK.pdf | 349KB | download |