Potentiation and inhibition of secretion from neutrophils by phorbol ester

FEBS Letters

Barrowman, M.M.¹  Gomperts, B.D.¹  Cockcroft, S.¹ 

[1] Department of Experimental Pathology, University College London, University Street, London WC1E 6JJ, England

关键词: Phorbol ester;    Ca2+;    fMetLeuPhe;    Neutrophil;    Exocytosis;    Protein phosphorylation;    Phosphatidate;    PDA;    4α-phorbol 12;    13-didecanoate;    PMA;    4β-phorbol 12β-myristate 13α-acetate;    IP3;    inositol 1;    4;    5-trisphosphate;    DG;    diacylglycerol;    fMet;    for-mylmethionyl;    PBS;    NaCl (0.154 M) containing 10 mM Na phosphate (pH 7);   

DOI  :  10.1016/0014-5793(86)80586-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We demonstrate that PMA exhibits multiple effects on fMetLeuPhe and ionophore-mediated secretion from rabbit and human neutrophils: (i) Inhibition of receptor-mediated secretion appears to take place at an early stage in the stimulus-secretion coupling sequence since PMA inhibits the formation of phosphatidate. (ii) Protein phosphorylation is observed with both PMA and fMetLeuPhe and the extent of phosphorylation is additive in the presence of both stimuli, (iii) Another possible site of regulation by PMA lies distal to the rise in cytosolic Ca²⁺. This is indicated by the enhancement or inhibition by PMA of the A23187-induced secretory response at low and high external Ca²⁺ concentrations, respectively.

【 授权许可】

Unknown