Adenylate cyclase of bovine adrenal cortex plasma membranes

FEBS Letters

de Foresta, Béatrice¹  Rogard, Monique¹  Gallay, Jacques¹ 

[1] Centre National de la Recherche Scientifique (ER 64-01), Unité d'Enseignement et de Recherche Biomédicale des Saints Pères, 45, rue des Saints Pères, 75270 Paris Cedex 06, France

关键词: Adenylate cyclase;    Forskolin;    Corticotropin;    Fluoride;    Adrenal cortex;    R;    receptor;    Gs;    stimulatory regulatory GTP-binding protein;    C;    catalytic unit of adenylate cyclase;    PMSF;    phenylmethylsulfonyl fluoride;    DMSO;    dimethyl sulfoxide;    DTE;    dithioerythritol;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(87)80766-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The diterpene forskolin maximally stimulated bovine adrenal cortex adenylate cyclase activity 9-fold with a concentration producing half-maximum effect (ED₅₀) of about 4 μM. The effects of forskolin and the fully active corticotropin fragment ACTH (1–24) were additive over nearly the whole range of concentration of both effectors, indicating separate and independent mechanisms of action. By contrast, 10 mM NaF blocked forskolin action in the nanomolar range of the diterpene concentration, while it allowed a partial stimulation by forskolin in the micromolar range. NaF thus reveals a heterogeneity of forskolin action in the adrenal cortex plasma membranes. Moreover, our data suggest that ACTH and NaF activation effects, both mediated by the stimulatory regulatory protein G_s, proceed through different mechanisms.

【 授权许可】

Unknown