Expression of ricin A chain in Escherichia coli

FEBS Letters

O'Hare, Mary²  Prior, Bernadette²  Watson, Graham J.¹  Thorpe, Philip E.¹  Roberts, Lynne M.²  Lord, J.Michael² 

[1] Drug Targeting Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, England;Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England

关键词: Ricin A chain;    Expression;    Immunotoxin;    (E.coli);   

DOI  :  10.1016/0014-5793(87)80759-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter P_N25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

【 授权许可】

Unknown