Biological activity of recombinant Ricinus communis agglutinin A chain produced in Escherichia coli

FEBS Letters

O'Hare, Mary¹  Roberts, Lynne M.¹  Lord, J.Michael¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

关键词: Ricinus communis agglutinin A chain;    Ricin A chain;    Expression;    E. coli;   

DOI  :  10.1016/0014-5793(92)80116-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA encoding Ricinus communis agglutinin A chain was listed into the E. coli expression vector pDS S/3. Induced E. coli 71.18 cells which had been transformed with this plasmid express Ricinus communis agglutinin A chain in a soluble and biologically active form. Recombinant Ricinus communis agglutinin A chain had ribosomal RNA N-glycosidase activity and was approximately 10-fold less active than ricin A chain in a cell-free protein synthesis inhibition assay.

【 授权许可】

Unknown