Expression of ricin B chain in Escherichia coli

FEBS Letters

Bowler, Christopher¹  Hussain, Khalid¹  Roberts, Lynne M.¹  Lord, J.Michael¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England

关键词: Ricin B chain;    Expression;    Lectin;    Immunotoxin;    (E. coli);   

DOI  :  10.1016/0014-5793(89)80568-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.

【 授权许可】

Unknown