The effect of pH on the conformation and stability of the structure of plant toxin — ricin

FEBS Letters

Tonevitsky, A.G.¹  Bushueva, T.L.¹ 

[1] Institute of Experimental Cardiology, Cardiology Research Center of the USSR Academy of Medical Sciences, 3rd Cherepkovskaya Street 15a, Moscow 121552, USSR

关键词: Ricin;    Protein fluorescence;    pH effect;    Guanidine hydrochloride;   

DOI  :  10.1016/0014-5793(87)80132-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of pH on the conformation of ricin and its A- and B-chains has been studied by measuring their intrinsic fluorescence. At pH 5.0 and 7.5, the structural stability of toxin and subunits was estimated according to the denaturing action of guanidine hydrochloride. It was demonstrated that the fluorescence of native toxin and catalytic A-subunit does not depend significantly on pH in the range pH 3–8, whereas ricin B-chain undergoes a structural transition at pH < 5.0. The structural stability of ricin and isolated chains differs significantly at pH 7.5 and 5.0; the structural stability of ricin and the A-chain increases, whereas that of the B-chain decreases.

【 授权许可】

Unknown