A 1H spin echo NMR study of the HeLa tumour cell

FEBS Letters

Al-Kabban, M.²  Stewart, M.J.²  Suckling, C.J.¹  Smith, W.E.¹  Reglinski, J.¹  Watson, I.D.² 

[1] Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL Scotland;Drug Investigation Unit, Department of Biochemistry, The Royal Infirmary, Glasgow G4 0SF, Scotland

关键词: Glycolysis;    HPLC;    Cancer;    Spin echo;    1H-NMR;    (HeLa cell);   

DOI  :  10.1016/0014-5793(87)80086-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylcholine, phosphorylcreatine, lactate, glutathione, glycine and leucine were identified in living HeLa cells in the first study of this cell type by ¹H spin echo NMR spectroscopy. The technique has the advantage that it is non-invasive, providing detailed structural information on individual species present in the cell matrix. It has been used in this case to study the rate of energy consumption following the activation of the glycolytic pathway with glucose.

【 授权许可】

Unknown