The norepinephrine‐sensitive Ca2+ ‐storage site differs from the caffeine‐sensitive site in vascular smooth muscle of the rat aorta

FEBS Letters

Kanaide, Hideo¹  Nakamura, Motoomi¹  Shogakiuchi, Yoshito¹ 

[1] Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan

关键词: Ca2+ store;    Norepinephrine;    Caffeine;    Quin 2;    (Vascular smooth muscle cell;    Rat aorta);    [Ca2+]i;    intracellular free calcium concentration;    PSS;    physiological saline solution;    VSMCs;    vascular smooth muscle cells;    NE;    norepinephrine;   

DOI  :  10.1016/0014-5793(87)80027-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using microfluorometry of quin 2, a Ca²⁺-sensitive dye, we characterized the release and uptake of Ca²⁺ by the norepinephrine-sensitive Ca²⁺-storage site and the caffeine-sensitive one. The norepinephrine-sensitive Ca²⁺-storage site was readily depleted in Ca²⁺-free medium and almost completely replenished by loading with 1.0 mM Ca²⁺ solution for 3 min, whereas the caffeine-sensitive site was scarcely affected. Furthermore, norepinephrine has little effect on the caffeine-sensitive Ca²⁺-storage site in Ca²⁺-free medium, and vice versa. We conclude that the location and mechanisms of release and uptake of Ca²⁺ of these two Ca²⁺-storage sites differ in the case of rat aortic vascular smooth muscle cells in primary culture.

【 授权许可】

Unknown