Cloning and expression of cDNA encoding human basic fibroblast growth factor

FEBS Letters

Iwane, Makoto¹  Kurokawa, Tsutomu¹  Sasada, Reiko¹  Igarashi, Koichi¹ 

[1] Biotechnology Laboratories of Central Research Division, Takeda Chemical Industries, Ltd, Yodogawaku, Osaka 532, Japan

关键词: Fibroblast growth factor;    cDNA;    mRNA;    Secretion;    (COS cell);   

DOI  :  10.1016/0014-5793(87)81489-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding human basic fibroblast growth factor (bFGF) was isolated from a human foreskin fibroblast cDNA library. The cDNA, 4 kilobases in size, had a coding sequence, 5' and 3' untranslated regions, and a poly(A) chain. Isolation of additional cDNA clones that had a short 3' untranslated region suggested the presence of multiple mRNA forms. By Northern blot analysis, at least five bFGF mRNA species were detected in cultured fibroblast cells. Transfection of the cDNA to COS cells resulted in the detection of mitogenic activity in the culture medium of the transfected cells, suggesting that a part of the synthesized protein might be secreted from cells despite its unusual short signal sequence.

【 授权许可】

Unknown