Secretion of human insulin by a transformed yeast cell

FEBS Letters

Hansen, Mogens T.¹  Thim, Lars¹  Sørensen, Anders R.¹ 

[1] Novo Research Institute, Novo Allé, DK-2880 Bagsvaerd, Denmark

关键词: Proinsulin;    C-peptide;    Processing;    Dibasic sequence;    Hormone precursor;   

DOI  :  10.1016/0014-5793(87)81366-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A yeast expression plasmid encoding a mini-proinsulin molecule was constructed and transformed into Saccharomyces cerevisiae. The plasmid encoded the sequence: B-Arg-Arg-Leu-Gln-Lys-Arg-A in which B represents the B-chain (30 amino acid residues) and A represents the A-chain (21 amino acid residues) of human insulin. The secreted peptides were shown to be a mixture of human insulin and des(B-30)human insulin. Thus, correct disulphide bridges can be established in proinsulin-like molecules devoid of a normal C-peptide region. Furthermore, the specificity of the yeast processing enzymes is so similar to the proinsulin converting enzymes in the human pancreatic β-cell that it allows the processing of the mini-proinsulin to insulin.

【 授权许可】

Unknown