Carboxypeptidase activity in the insulin secretory granule

FEBS Letters

Hutton, John C.¹  Docherty, Kevin¹ 

[1] Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, England

关键词: Proinsulin Processing Carboxypeptidase Granule Insulinoma Prohormone;   

DOI  :  10.1016/0014-5793(83)81065-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carboxypeptidase activity was studied in subcellular fractions from a transplantable rat insulinoma and found to be localised principally in the insulin secretory granule. The activity, which was specific for peptide substrates with C-terminal basic amino acids, appeared to be a single enzyme with M _r 54 000. This enzyme differed with respect to size and pH optimum from other basic amino acid-specific carboxypeptidases, such as carboxypeptidases B and N, and may be a secretory granule-specific enzyme involved in propolypeptide processing.

【 授权许可】

Unknown