The secretion of glucagon by transformed yeast strains

FEBS Letters

Thim, L.¹  Norris, F.¹  Hansen, M.T.¹  Moody, A.J.¹  Norris, K.¹ 

[1] Novo Research Institute, Novo Allé, DK-2880 Bagsvaerd, Denmark

关键词: Recombinant glucagon secretion;    (Yeast);    MeCN;    acetonitrile;    GIP;    gastric inhibitory peptide;    VIP;    vasoactive intestinal peptide;    GLI;    glucagon-like immunoreactivity;   

DOI  :  10.1016/0014-5793(87)81365-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Saccharomyces cerevisiae strains were transformed with plasmids coding for modified mating factor α₁ leader sequences followed by glucagon. Glucagon-containing peptides which were secreted into the fermentation broth were isolated and their amino acid sequences determined. The yeast strain transformed with the sequence coding for the complete mating factor α₁ leader sequence preceding the glucagon gene (MT556) secreted glucagon plus glucagon extended at its N-terminal by parts of the leader sequence. The yeast strain transformed with the sequence coding for a truncated mating factor α₁ leader sequence before the glucagon gene (MT615) secreted glucagon. These observations suggest that S. cerevisiae is a suitable vehicle for the efficient expression of plasmids coding for polypeptides similar to glucagon (e.g. VIP, secretin, GIP).

【 授权许可】

Unknown