A plausible mechanism for large‐scale chromosomal DNA amplification in streptomycetes

FEBS Letters

Cullum, John²  Young, Michael¹ 

[1] Department of Botany and Microbiology, University College of Wales, Aberystwyth SY23 3D A, Wales, England;Department of Biochemistry and Applied Molecular Biology, UMIST, PO Box 88, Manchester M60 1QD, England

关键词: Gene amplification;    Amplification mechanism;    (Streptomycetes);   

DOI  :  10.1016/0014-5793(87)81547-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recent advances in our understanding of the structure of highly amplified DNA sequences in Streptomyces fradiae and lividans have enabled us to formulate a possible mechanism by which amplification may occur. An essential feature of the model is the generation of an amplification precursor, which comprises a circularised copy of the DNA to be amplified, attached to one arm of the chromosome by a replication fork. Multiple copies of the amplifiable DNA are generated by rolling circle replication. The model adequately accounts for many features of gene amplification in these two species, including the tendency for deletions to occur to one side, but not the other, of the amplified DNA.

【 授权许可】

Unknown