Structure determination of the O‐specific polysaccharides from Citrobacter O4‐ and O27‐lipopolysaccharides by methylation analysis and one‐ and two‐dimensional 1 H‐NMR spectroscopy

FEBS Letters

Dabrowski, Janusz¹  Romanowska, Anna¹  Romanowska, Elżbieta¹  Hauck, Michael¹ 

[1] Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland

关键词: Structure determination;    O-specific polysaccharide;    Methylation analysis;    1H-NMR;    (Citrobacter freundii);    COSY;    two-dimensional scalar shift-correlated NMR spectrum;    D and 2D;    one- and two-dimensional;    NOE;    nuclear Overhauser effect;   

DOI  :  10.1016/0014-5793(87)81431-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using sugar and methylation analyses, and one- and two-dimensional ¹H-NMR spectroscopy at 500 MHz it was established that poly-β-1,2-4-deoxy-D-arabinohexopyranose occurs as O-specific chains of lipopolysaccharides in Citrobacter serotypes O4, O27 and O36. Strong serological cross-reactivity between these serotypes is in full agreement with the chemical identity of their O-specific polysaccharides.

【 授权许可】

Unknown