Absence of a unique relationship between active transport of lactose and protonmotive force in E. coli

FEBS Letters

Shechter, Emanuel¹  Delamourd, Lourdes¹  Ghazi, Alexandre¹ 

[1] Laboratoire des Biomembranes (UA 1116), Batiment 432, Université de Paris-Sud, 91405 Orsay, France

关键词: Active transport;    Lactose permease;    Protonmotive force;    Chemiosmotic theory Flow-force relationship (E. coli);    TPP;    tetraphenylphosphonium ion;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    TCS;    3;    3';    4';    5-tetrachlorosalicylanilide;    PCMBS;    p-chloromercuribenzenesulfonate;    Δp;    protonmotive force;    ΔpH;    transmembrane pH difference;    Δψ;    transmembrane electrical potential difference;    Δplac;    (RT/F)·ln([lactose]in/[lactose]out);   

DOI  :  10.1016/0014-5793(86)81136-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized chemiosmotic theory, two different relationships were obtained depending on the method used.

【 授权许可】

Unknown