A shift in chloroplast energy coupling by KCl from localized to bulk phase delocalized proton gradients

FEBS Letters

Dilley, Richard A.¹  Beard, William A.¹ 

[1] Department of Biological sciences, Purdue University, West Lafayette, IN 47906, USA

关键词: ATP synthesis;    Protonmotive force;    salt effect;    Single-turnover flash;    Thylakoid membrane;    Tricine;    N-tris(hydroxymethyl)methylglycine;    CF0-CF1;    integral membrane and peripheral portions of the coupling factor complex;    respectively;    DTT;    dithiothreitol;    Chl;    chlorophyll;   

DOI  :  10.1016/0014-5793(86)80570-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spinach thylakoids were resuspended in a buffer including either 200 mM sucrose or 100 mM KCl. singleturnover flash excitation and the luciferin-luciferase system were used to energize and follow ATP formation. The effect of the permeable buffer pyridine was measured on the ATP formation onset lag and on the post-illumination ATP yield. Consistent with a bulk phase delocalized proton gradient coupling model, thylakoids stored in 100 mM KCl exhibited an increase in these two parameters in the presence of pyridine. Thylakoids isolated in the absence of KCl showed no effect of pyridine on the two parameters indicating a localized energy coupling mode.

【 授权许可】

Unknown