ATP synthesis with single turnover flashes in spinach chloroplasts

FEBS Letters

Schreiber, U.¹  Del Valle-Tascon, S.¹ 

[1] Lehrstuhl für Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, 8700 Würzburg, FRG

关键词: ATP synthesis;    ATP hydrolysis;    Single-turnover flash;    Thylakoidal ATPase;    Coupling mechanism;    Hepes;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;    Tris;    tris-(hydroxymethyl)-aminomethane;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    TNBT;    tri-n-butyltin;    CF1;    chloroplast coupling factor;    chl;    chlorophyll;   

DOI  :  10.1016/0014-5793(82)81299-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single-turnover flash-induced ATP synthesis in chloroplasts was measured in situ with the luciferin luminescence method. In dark-adapted chloroplasts the first flashes only induce ATP hydrolysis. Once the reversible ATPase is fully activated, ATP hydrolysis persists for extended periods of darkness and flash-induced ATP-synthesis is optimal even at flash frequencies lower than 0.1 Hz. About one molecule of ATP is formed per 1000 chlorophyll and flash. In a low frequency flashing regime under steady state conditions, the newly formed ATP is stable. There is no threshold light intensity for flash-induced ATP synthesis. The data are in agreement with models involving short-range interaction between electron transport and the coupling factor.

【 授权许可】

Unknown