Discrimination between transmembrane ion gradient‐driven and electron transfer‐driven ATP synthesis in the methanogenic bacteria

FEBS Letters

Lancaster, J.R.¹  Al-Mahrouq, H.A.¹  Carper, S.W.¹ 

[1] Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322, USA

关键词: Methanogen;    Bioenergetics;    Na+ pump;    ATPase;   

DOI  :  10.1016/0014-5793(86)81501-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As with Methanococcus voltae [(1986) FEBS Lett. 200, 177–180], ATP synthesis in Methanobacterium thermoautotrophicum (ΔH) can be driven by the imposition of a sodium gradient, but only in the presence of a counterion. Monensin (but not SF6847) inhibits this synthesis. Methanogenic electron transfer-driven ATP synthesis, however, is insensitive to the combination of these two ionophores. In M. voltae, 117 μM diethylstilbestrol effectively inhibits both membrane potential- and sodium gradient-driven ATP synthesis, but has no effect on ATP production coupled to methanogenesis. In Mb. thermoautotrophicum (ΔH), a similar pattern of inhibition is exhibited by harmaline, an inhibitor of sodium-linked membrane transport systems. We conclude that ATP-driven sodium translocation and electron transfer-driven ATP synthesis are accomplished by separate entities, at least for these two only distantly related species of methanogen.

【 授权许可】

Unknown