Synthesis of human des‐tryptophan‐1,norleucine‐12‐minigastrin‐II and its biological activities

FEBS Letters

Calderan, A.²  Wünsch, E.²  Borin, G.²  Göhring, W.¹  Moroder, L.¹  Bali, J.-P.³ 

[1] Max-Planck-Institut für Biochemie, Abteilung Peptidchemie, 8033 Martinsried, FRG;Centro di Studi sui Biopolimeri, Istituto di Chimica Organica, Universitá di Padova, Padova, Italy;ER-CNRS 228, École nationale supérieure de Chemie, 34075 Montpellier, France

关键词: Gastrin;    Sulfation;    Peptide synthesis;    Gastric acid secretion;   

DOI  :  10.1016/0014-5793(86)80981-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human minigastrin-II analog was prepared by conventional methods in solution using N-benzyloxycarbonyltyrosine O-sulfate as starting material in the synthetic route. Upon removal of the acid-labile protecting groups and purification by preparative reversed-phase chromatography the sulfated gastrin peptide was obtained in satisfactory overall yields as homogeneous material. It was found to be about twice as active as the non-sulfated form in stimulating gastric acid secretion and to exhibit a 10-fold higher affinity to gastrin receptors of purified parietal cells.

【 授权许可】

Unknown