Thrombin and IgE antigen induce formation of inositol phosphates by mouse E‐mast cells

FEBS Letters

Liu, F.T.¹  Razin, E.²  Baranes, D.² 

[1] Department of Immunology, The Medical Biology Institute, La Jolla, CA 92037, USA;Institute of Biochemistry, Hebrew University Medical School, PO Box 1172, Jerusalem 91010, Israel

关键词: (E-mast cell);    Inositol phosphate;    GPI;    glycerophosphatidylinositol;    IgE;    immunoglobulin E;    IPs;    inositol phosphates;    IP2;    inositol 1;    4-bisphosphate;    IP3;    inositol 1;    4;    5-trisphosphate;    PI;    phosphatidylinositol;    TG;    Tyrode's buffer;   

DOI  :  10.1016/0014-5793(86)81341-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of murine chondroitin sulfate E containing mast cells (E-MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP₁ IP₂ and IP₃) could be detected 20 s after stimulation. The depletion of Ca²⁺ from the medium resulted in more than 80% reduction in β-hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP₃ under these conditions independent of the presence of extracellular Ca²⁺.

【 授权许可】

Unknown