Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae

FEBS Letters

Saiardi, Adolfo¹  Caffrey, James J²  Shears, Stephen B²  Snyder, Solomon H¹ 

[1] Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;Inositide Signaling Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA

关键词: ARGRIII;    Inositol polyphosphate multikinase;    Inositol phosphate;    InsP6;    mRNA export;    Yeast;   

DOI  :  10.1016/S0014-5793(00)01194-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al. (1999) Curr. Biol. 9, 1323–1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP₃] 170-fold and decreased [InsP₆] 100-fold, indicating reduced phosphorylation of InsP₃ to InsP₆. Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP₆. Low levels of InsP₆, and considerable quantities of Ins(1,3,4,5)P₄, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism.

【 授权许可】

Unknown