Mitogen treatment of permeabilized human T lymphocytes stimulates rapid tyrosine and serine phosphorylation of a 42 kDa protein

FEBS Letters

Hoffbrand, A.Victor¹  Mire, Anthony R.¹  Wickremasinghe, R.Gitendra¹ 

[1] Department of Haematology, Royal Free Hospital, Pond Street, Hampstead, London NW3 2QG, England

关键词: T lymphocyte;    Tyrosine protein kinase;    Protein kinase C;    Mitogenesis;    PHA;    phytohaemagglutinin;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(86)81339-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three agents which are mitogenic for T lymphocytes (phytohaemagglutinin, monoclonal antibody UCHT 1 and 12-O-tetradecanoylphorbol-13-acetate) stimulated rapid phosphorylation of a 42 kDa protein in per-meabilized T lymphocytes. Phosphorylation occurred on tyrosine and serine residues. A non-mitogenic monoclonal antibody (RFT11) did not stimulate phosphorylation of this protein. Furthermore, the dose response of 42 kDa protein phosphorylation and of mitogenesis to increasing amounts of phytohaemagglutinin were closely similar. We therefore propose that mitogen-stimulated phosphorylation of the 42 kDa protein is part of the mechanism for transduction of mitogenic signals in lymphocytes. To our knowledge, this is the first report of rapid, ligand-stimulated tyrosine protein phosphorylation in T lymphocytes.

【 授权许可】

Unknown