Quaternary structure of the liver microsomal cytochrome P‐450

FEBS Letters

Archakov, A.I.¹  Orlova, E.V.¹  Myasoedova, K.N.¹  Chernyak, V.Ya.¹  Sograf, O.N.¹  Tsuprun, V.L.¹  Skulachev, V.P.¹  Berndt, P.¹ 

[1] Shubnikov Institute of Crystallography, USSR Academy of Sciences Moscow State University, Moscow 119899, USSR

关键词: Cytochrome P-450;    Quaternary structure;    Electron microscopy;   

DOI  :  10.1016/0014-5793(86)80861-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytochrome P-450_LM2 was isolated from rabbit liver microsomes in a form which was shown to be homogeneous in AcA-22 Ultrogel and ultracentrifugation studies. The molecular mass determined by sedimentation equilibrium roughly corresponded to hexamer composed of 56 kDa monomers. Hexamer structure of the cytochrome was directly demonstrated by electron microscopic study. In the cytochrome P-450_LM2 hexamer, monomers seem to be arranged in two layers (three monomers in the layer) in such a way that each monomer occupies aposition at the vertices of a triangular antiprism with a 32 point group symmetry.

【 授权许可】

Unknown