Quaternary organization of subunits in the L‐leucine dehydrogenase from Bacillus cereus

FEBS Letters

Lünsdorf, Heinrich¹  Tsai, Hsin¹ 

[1] GBF, Gesellschaft für Biotechnologische Forschung mbH, D-3300 Braunschweig-Stöckheim, FRG

关键词: Leucine dehydrogenase;    Electron microscopy;    Submit stoichiometry;    Quaternary structure;    (Bacillus cereus);   

DOI  :  10.1016/0014-5793(85)80165-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

L-Leucine dehydrogenase from Bacillus cereus was examined in the electron microscope. The quaternary structure reveals a molecule that is built up from 8 subunits, identical in mass, arranged in 2 layers which are oriented mainly in a staggered form. In each layer subunits are positioned at the vertices of a square, leaving free a central protein-deficient region of 2.6 nm in diameter. The enzyme measures 11.1 nm in diagonal and 9.0 nm in edge length. Mean subunit diameter is 4.0 nm. The overall shape is a cube, slightly compressed, with 90% edge length in height.

【 授权许可】

Unknown