Are microtubules cold‐stable in the Atlantic cod, Gadus morhua?

FEBS Letters

Wallin, M.¹  Jönsson, A.-C.¹  Strömberg, E.¹ 

[1] Department of Zoophysiology, Comparative Neuroscience Unit, University of Göteborg, Box 250 59, S-400 31 Göteborg, Sweden

关键词: Microtubule;    Cold stability;    Cold lability;    Axonal transport;    Microtubule-associated protein;   

DOI  :  10.1016/0014-5793(86)81396-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amount of axonally transported proteins in the nervus splanchnicus of cod (Gadus morhua) was found to be temperature-dependent in vitro, with an optimum at 8°C. The transport was markedly reduced at 2°C, probably caused by decreased protein synthesis rather than disassembly of microtubules. Microtubules were isolated from cod brain by cycles of assembly-disassembly. These microtubules were cold-labile, had a low amount of microtubule-associated proteins and a high critical concentration for assembly. The possibilities exist that a cold-stabilizing factor or cold-stable microtubules are lost during the preparation, or that cold-stable microtubules are components of the peripheral axons only.

【 授权许可】

Unknown