Differences in polypeptide composition and enzyme activity between cold‐stable and cold‐labile microtubules and study of microtubule alkaline phosphatase activity

FEBS Letters

Hesketh, J.E.¹ 

[1] Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, Scotland

关键词: Microtubule;    Cold stability;    Microtubule-associated protein;    SDS-gel electrophoresis;    Alkaline phosphatase;    Zinc;   

DOI  :  10.1016/0014-5793(84)80341-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable preparations showed a much higher alkaline phosphatase activity. Stimulation of microtubule assembly by zinc led to increases in both cold stability and alkaline phosphatase activity.

【 授权许可】

Unknown