FEBS Letters | |
Low temperature persistence of type I antifreeze protein is mediated by cold‐specific mRNA stability | |
Koops, M.Derek2  Davies, Peter L.1  Duncker, Bernard P.1  Walker, Virginia K.1  | |
[1] Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada;Department of Biochemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada | |
关键词: Antifreeze protein; mRNA turnover; Cold stability; Transgenic Drosophila; Heat shock; | |
DOI : 10.1016/0014-5793(95)01340-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In winter flounder, the levels of type I antifreeze protein (AFP) and its mRNA vary seasonally by as much as 1000-fold. Elevated levels in the fall are prompted by the loss of long day-lengths, while higher spring temperatures correlate with AFP clearance. We have investigated the role of temperature on AFP accumulation using transgenic Drosophila melanogaster by expressing multiple AFP genes under control of the heat-inducible hsp70 promoter. AFP and AFP mRNA persisted far longer in files reared at 10°C compared to 22°C. This difference appears to be mediated by cold-specific mRNA stability since no such temperature effect was observed with either an endogenous heat-inducible mRNA or a constitutively expressed mRNA.
【 授权许可】
Unknown
【 预 览 】
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RO201912020302055ZK.pdf | 481KB | download |