Leukotriene B4 stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin

FEBS Letters

Holian, Andrij¹ 

[1] Pulmonary Division of the Department of Internal Medicine and Department of Pharmacology, University of Texas Medical school, Houston, TX 77030, USA

关键词: Macrophage;    Formyl peptide;    Leukotriene B4;    Phosphatidylinositol;    Pertussis toxin;   

DOI  :  10.1016/0014-5793(86)80562-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of leukotriene B₄ (LTB₄) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966-5969], LTB₄ was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate, transient elevation of 1,2-diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB₄ and formyl peptide stimulated O₂ ⁻ production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.

【 授权许可】

Unknown