Tick‐borne encephalitis virus genome

FEBS Letters

Blinov, V.M.²  Pletnev, A.G.¹  Yamshchikov, V.F.¹ 

[1] Novosibirsk Institute ofBioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 90 USSR;All-Union Research Institute of Molecular Biology, Koltsovo, Novosibirsk District, USSR

关键词: Encephalitis virus;    cDNA cloning;    Nucleotide sequence;    Viral protein;    Protein structure;   

DOI  :  10.1016/0014-5793(86)81160-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNA of a flavivirus, tick-borne encephalitis virus (TBEV; strain Sofjin), was subjected to reverse transcription and the DNA copy was transformed into double-stranded DNA by the action of E. coli DNA-polymerase I (Klenow fragment). This DNA was annealed with plasmid pBR322. The recombinant plasmids were cloned in E. coli K802. The nucleotide sequence of the inserts of the clones, coding for region structural proteins C, M, E and nonstructural protein NS1, was determined by the Maxam-Gilbert method. The genes of structural proteins form a compact cluster. Homology has been studied of the TBEV sequences found with the structures of proteins and RNAs of other flaviviruses, yellow fever virus and West Nile virus, and a high degree of homology was found.

【 授权许可】

Unknown