Stereospecific antibodies to propranolol

FEBS Letters

Levitzki, Alexander²  Feingers, Judith³  Inbar, Michael³  Wang, Lynn³  Chorev, Michael¹ 

[1] Department of Pharmaceutical Chemistry, School of Pharmacy, The Hebrew University of Jerusalem, 91120 JerusalemIsrael;Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel;International Diagnostic Laboratories, PO Box 5577, Jerusalem, Israel

关键词: Propranolol;    Monoclonal antibody;    Stereospecificity;    β-Adrenergic receptor;    β-Adrenergic antagonist;   

DOI  :  10.1016/0014-5793(86)80474-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The β-adrenergic antagonist propranolol was activated through its side chain, coupled to bovine serum albumin, and injected into BALB/c mice. After fusion of the splenocytes from these immunized mice with the NS-1 myeloma cell line, two hybridomas, producing monoclonal anti-propranolol antibodies, were isolated. Clone P-49 was monospecific for propranolol, with a significant preference for the 1-stereoisomer, as compared to the d form. On the other hand, clone P-28 cross-reacted with alprenolol as well as some other β-antagonists. Both classes of antibodies competed with A431 epidermoid carcinoma β₂-adrenoceptors for the binding of [³H]propranolol. When ascites cells from clone P-28 were fixed with glutaraldehyde, the anti-propranolol monoclonal antibody became cell bound. These cell-bound P-28 antibodies bind propranolol and other β-adrenergic ligands with a similar ranking order to the soluble monoclonal antibody. The cell-bound antibody displayed a 5-fold higher affinity towards 1-propranolol than the soluble monoclonal antibody. The practical implications of these findings are discussed.

【 授权许可】

Unknown