Changes in the guanine nucleotide‐binding proteins, Gi and go, during differentiation of 3T3‐L1 cells

FEBS Letters

Spiegel, Allen³  Rubin, Charles¹  Gierschik, Peter⁴  Milligan, Graeme²  Morrow, Bernice¹ 

[1] Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA;Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA;Metabolic Diseases Branch, National Institute of Arthritis, Diabetes, Digestive, and Kidney Disease, Albert Einstein College of Medicine, Bronx, NY 10461, USA;Present address: Dept of Pharmacology, University of Heidelberg, Heidelberg, FRG.

关键词: Pertussis toxin;    Adenylate cyclase;    Signal transduction;    G-protein;    guanine nucleotide-binding protein;    Gs and Gi;    the G-proteins associated with stimulation and inhibition;    respectively;    of adenylate cyclase;    Go;    a G-protein of unknown function discovered in brain;    DEX + IBMX;    dexamethasone + isobutylmethylxanthine;   

DOI  :  10.1016/0014-5793(86)81233-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Differentiation of 3T3-L1 cells from fibroblasts to adipocytes is accompanied by increased adenylate cyclase response to lipolytic agents. We used pertussis toxin and specific antibodies to measure the inhibitory guanine nucleotide-binding protein, G_i, and the novel G-protein, G_o, in membranes from 3T3-L1 cells. Pertussis toxin-dependent labeling of a 39–40 kDa protein showed an initial 30% rise, followed by an 80% fall during differentiation. Immunoblots showed that 3T3-L1 cells contain G_o, as well as G_i, and that changes in the former parallel the changes in pertussis toxin labeling. Changes in G_i and G_o may contribute to altered adenylate cyclase response during 3T3-L1 cell differentiation.

【 授权许可】

Unknown