The branched respiratory chain of heterotrophically dark‐grown Chloroflexus aurantiacus

FEBS Letters

Zannoni, Davide¹ 

[1] Institute of Botany, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy

关键词: Cytochrome;    a Cytochrome a3;    Cytochrome;    b Cytochrome c;    Respiration;    Thermophilic bacterium (Chloroflexus aurantiacus);   

DOI  :  10.1016/0014-5793(86)81196-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The respiratory electron-transport chain of heterotrophically dark-grown Chloroflexus aurantiacus has been investigated. Membranes isolated from these cells have been shown to contain at least three c-type cytochromes (E _{m, 7.0} 255,180, and 10 mV), three b-type cytochromes (E _{m, 7.0} of 210, 60 and −65 mV) and two cytochromes of the a type with E _{m, 7.0} of 330 and 190 mV. Spectroscopic evidence from CO-difference spectra, CN⁻-duference spectra and spectra at fixed oxidation-reduction potentials suggests that the two a-type components may be analogous to cytochromes a and a ₃ of mitochondria. The analyses of the effects induced by CN⁻, myxothiazol and antimycin A on both steady-state respiratory activities and semi-rapid oxidation-reduction kinetic patterns of c- and a-type cytochromes indicate the presence of a branched respiratory chain. Growth of Chloroflexus in medium lacking added copper diminished the concentration of the a-type cytochromes but not those of cytochromes of the b and c type.

【 授权许可】

Unknown