Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle

BMB Reports

Seung-Hyeob Kim^1¹  Jae-Seon Lee^2²  Hyun Lee^1³ 

[1] Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 22212^2;Department of Chemistry, Seoul National University, Seoul 08826, Korea^3;Division of Life Sciences, Korea University, Seoul 02841^1

关键词: Cytochrome c;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.

【 授权许可】

Unknown