Comparison of the effects of bax‐expression in yeast under fermentative and respiratory conditions: investigation of the role of adenine nucleotides carrier and cytochrome c

FEBS Letters

Priault, Muriel¹  Schaeffer, Jacques¹  Manon, Stéphen¹  Chaudhuri, Bhabatosh²  Camougrand, Nadine¹ 

[1] Institut de Biochimie et Génétique Cellulaires du C.N.R.S., 1 rue Camille Saint-Saëns, 33077 Bordeaux, France;Oncology Research, Novarits-Pharma A.G., K-125.13.17, Basel, Switzerland

关键词: bax-Expression;    Adenine nucleotides carrier;    Cytochrome c;    ANC;    adenine nucleotides carrier;    PTP;    permeability transition pore;    VDAC;    voltage-dependent anion channel;   

DOI  :  10.1016/S0014-5793(99)00957-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new system for bax-expression in yeast has been devised to investigate bax's effect under fermentative and respiro-fermentative conditions. This has allowed us to show unambiguously that the ability of bax to kill yeast is higher under respiratory conditions than under purely fermentative conditions. The extent of killing under respiro-fermentative conditions (non-repressive sugars) is intermediate. It has been proposed that the two proteins adenine nucleotides carrier (ANC) and cytochrome c play a crucial role in bax-induced cell death. We have investigated the effects of deletion of the genes encoding the two proteins on the toxicity induced by bax, using this new system. The absence of ANC did not modify bax-induced lethality in any way. Moreover, the absence of cytochrome c also did not prevent bax-induced death. Only the kinetics of lethality were altered. All these effects are prevented by co-expression of bcl-x _L.

【 授权许可】

Unknown