Reconstitution of photosynthetic electron transfer in cyanobacterial heterocyst membranes

FEBS Letters

Hawkesford, Malcolm J.¹  Houchins, Jeffrey P.¹  Hind, Geoffrey¹ 

[1] Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA

关键词: Anabaena;    Heterocyst;    Photosystem 1;    Reconstitution;    Cytochrome;    Plastocyanin;    chl;    chlorophyll;    cyt.;    cytochrome;    DCIP;    dichlorophenol-indophenol;    FNR;    ferredoxin: NADP oxidoreductase;    HEPES;    N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid;    HQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    PS I and II;    photosystems I and II;    PC;    plastocyanin;   

DOI  :  10.1016/0014-5793(83)80460-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosynthetic membranes have been isolated from heterocysts of the cyanobacterium Anabaena 7120. Electron transfer reactions associated with photosystem I have been investigated using single turnover flashes. Turnover rates, which were dependent on reconstitution with cytochrome c-553 or plastocyanin, were maximal when a suitable electron donor system was provided. Hydrogen (via an endogenous uptake hydrogenase), NADH (via a dehydrogenase) or the ascorbate/dichlorophenolindophenol couple were all effective donors. NADPH could also serve as a donor but this system was dependent on addition of ferredoxin and ferredoxin—NADP reductase. Turnovers of P700, soluble cytochrome c-553 and cytochrome b-563 could be demonstrated.

【 授权许可】

Unknown