Kinetic and spectral resolution of cytochrome c‐553 and cytochrome f in the photosynthetic electron‐transfer chain of heterocysts

FEBS Letters

Hawkesford, Malcolm J.¹  Houchins, Jeffrey P.¹  Hind, Geoffrey¹ 

[1] Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA

关键词: Heterocyst;    Cytochrome f;    Cytochrome c-553;    Plastocyanin;    Anabaena;    Photosynthetic electron transfer;   

DOI  :  10.1016/0014-5793(83)80461-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidation of cytochrome c-553 and cytochrome f following single-turnover flashes was measured in isolated heterocysts. Half-times of oxidation for these two cytochromes were determined by measuring the rate of bleaching at 554 nm in heterocysts possessing cytochrome c-553, or in heterocysts in which cytochrome c-553 was replaced by plastocyanin. Flash-induced difference spectra demonstrate that a fast bleaching (t _0.5 = 20–35 μs) corresponds to the oxidation of cytochrome c-553, and a slower bleaching (t _0.5 = 87 μs) corresponds to the oxidation of cytochrome f.

【 授权许可】

Unknown