Ruthenium red inhibits the mitochondrial Ca2+ uptake in intact bovine spermatozoa and increases the cytosolic Ca2+ concentration

FEBS Letters

Deana, Renzo¹  Rigoni, Fernanda² 

[1] Institute of Biological Chemistry, University of Padova and Unit of Consiglio Nazionale delle Ricerchefor the Study of the Physiology of Mitochondria, Via Marzolo 3, 35131 Padova, Italy;>Department of Biology University of Padova and Unit of Consiglio Nazionale delle Ricerche for the Study of the Physiology of Mitochondria, Via Marzolo 3, 35131 Padova, Italy

关键词: (Bovine spermatozoa);    Cytosolic Ca2+;    Mitochondria;    Ca2+transort;    Ruthenium red;    DTPA;    diethylenetriaminepentaacetic acid;    TPEN;    N;    N;    N';    N'-tetrakis(2-pyridylmethyl)-ethylenediamide;    RR;    ruthenium red;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(86)81193-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The uptake and cycling of Ca²⁺ by ejaculated bovine spermatozoa are almost completely abolished by ruthenium red, antimycin A or FCCP. The inhibitory effect of ruthenium red is also observed after washing of the dye-pretreated cells followed by addition of digitonin or filipin. In contrast, the inhibition is overcome by A23187 treatment. It is concluded that ruthenium red penetrates into intact spermatozoa, inhibits the mitochondrial Ca²⁺ uptake ‘in situ’, and causes the observed increase of the cytosolic free Ca²⁺ concentration.

【 授权许可】

Unknown