Amino acid sequence of human plasma apolipoprotein C‐III from normalipidemic subjects

FEBS Letters

Fairwell, Thomas¹  Hospattankar, Ashok V.¹  Ronan, Rosemary¹  Bryan Brewer, H.¹ 

[1] Molecular Disease Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Amino acid sequence Apolipoprotein C-III;    apo;    apolipoprotein;    VLDL;    very low density lipoproteins;    HDL;    high density lipoproteins;    HPLC;    high-performance liquid chromatography;    PTH;    phenylthiodantoin;   

DOI  :  10.1016/0014-5793(86)80300-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptides. The complete amino acid sequence of apoC-III was determined by the automated Edman degradation of the intact protein as well as the various tryptic peptides. Phenylthiohydantoin amino acids were identified by high-performance liquid chromatography and chemical ionization mass spectrometry. The amino acid sequence of apoC-III isolated from normalipidemic subjects is identical to the apoC-III sequence derived from the cDNA sequence and differs at 4 positions from the previously reported sequence of apoC-III derived from a patient with type V hyperlipoproteinemia.

【 授权许可】

Unknown