Amino acid sequence of heat‐stable enterotoxin produced by Vibrio cholerae non‐01

FEBS Letters

Nishimura, Osamu¹  Takeda, Tae³  Kobayashi, Makoto¹  Arita, Michiko³  Honda, Takeshi³  Shimonishi, Yasutsugu²  Takao, Toshifumi²  Miwatani, Toshio³ 

[1] Biotechnology Laboratories, Central Research Division, Takeda Chemical Industries Ltd, Yodogawa-ku, Osaka 532, Japan;Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan;Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan

关键词: (Vibrio cholerae non-01);    Heat-stability;    Enterotoxin;    Primary structure;   

DOI  :  10.1016/0014-5793(85)80163-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino acid sequence of heat-stable enterotoxin, produced by Vibrio cholerae non-01 and isolated from its culture supernatant, was determined by both Edman degradation of native and reductively carboxy-methylated enterotoxin and also a combination of fast atom bombardment mass spectrometry and carboxy-peptidase Y digestion of native enterotoxin to be as follows: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This sequence is very similar, but not identical, to those of heat-stable enterotoxins produced by enterotoxigenic Escherichia coli and Yersinia enterocolitica.

【 授权许可】

Unknown