Isolation of a cDNA clone for a catalytic subunit of Torpedo marmorata acetylcholinesterase

FEBS Letters

Massoulié, Jean²  Sikorav, Jean-Louis²  Grassi, Jacques¹  Valletta, François² 

[1] Laboratoire d'Etudes Radioimmunologiques, Section de Pharmacologie et d'Immunologie, Département de Biologie, Centre d'Etudes Nucléaires de Saclay, BP 2, 91190 Gif-sur-Yvette, France;Laboratoire de Neurobiologie Ecole Normale Supérieure 46 rue d'Ulm 75005 ParisFrance

关键词: Acetylcholinesterase;    cDNA cloning;    Expression screening;    (Torpedo marmorata);   

DOI  :  10.1016/0014-5793(85)80142-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have constructed a cDNA library from Torpedo marmorata electric organ poly(A⁺) RNA in the lambda phage expression vector λgtll. This library has been screened with polyclonal anti-acetylcholinesterase antibodies. One clone, λAChEl, produced a fusion protein which was recognized by the antibodies and which prevented the binding of native acetylcholinesterase in an enzymatic immune assay. These results indicate that λAChEl contains a cDNA insert coding for a part of a catalytic subunit of Torpedo acetylcholinesterase. The 200-base-pair cDNA insert hybridized to three mRNAs (14.5, 10.5 and 5.5 kb) from Torpedo electric organs. These mRNAs were also detected in Torpedo electric lobes.

【 授权许可】

Unknown