Cholesteryl ester transfer protein

FEBS Letters

Manent, Jeanine¹  Ierides, Michael¹  Dousset, Nicole¹  Potier, Michel²  Douste-Blazy, Louis¹  Carton, Michel³ 

[1]INSERM Unité 101, Hôpital Purpan, 31059 Toulouse, France

[2]Hôpital Ste Justine, 3175 Chemin Ste Catherine, Montreal, Canada

[3]Centre Claudius Regaud, Rue du Pont St Pierre, 31052 Toulouse, France

关键词: Cholesteryl ester transfer protein;    Radiation inactivation;    Target theory;    Mr Isoelectric point;    HDL;    high density lipoprotein;    LDL;    low density lipoprotein;    VLDL;    very low density lipoprotein;    DTNB;    dithiobisnitrobenzoic acid;    LPL;    lipoprotein lipase;   

DOI  :  10.1016/0014-5793(85)80079-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Radiation inactivation was used to determine the functional M _r of cholesteryl ester transfer protein (CETP) in rabbit plasma from control and irradiated animals. This technique reveals the size of the functional unit required to carry out the transfer function. The functional M _r was calculated to be 70000 ± 3000 (mean ± SD) for both control and irradiated rabbits. This result is in. accordance with the M _r obtained by a completely different method, namely SDS-polyacrylamide gel electrophoresis of a partially purified (110-fold) rabbit CETP. The pI of this CETP was found by isoelectric focusing to be equal to 5.95. The results suggest that the functional unit of this enzyme is the monomer.

【 授权许可】

Unknown