Molecular forms of glucagon‐like peptides in man

FEBS Letters

Uttenthal, L.O.¹  Bloom, S.R.¹  George, S.K.¹  Ghiglione, M.¹ 

[1] Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, London W12 OHS, England

关键词: Glucagon-like peptide;    Proglucagon;    Post-translational processing;    Pancreas;    Intestine;   

DOI  :  10.1016/0014-5793(85)80124-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Molecular forms of the glucagon-like peptides (GLP) encoded by the human preproglucagon gene were analysed by chromatography combined with specific radioimmunoassays to the synthetic peptides. Whereas extracts of human pancreas and a glucagonoma contained a large proglucagon cleavage product possessing both GLP-1 and GLP-2 immunoreactivities, extracts of human intestine contained products corresponding to free GLP-1 and a small amount ofchromatographically distinct GLP-2 immunoreactivity. It is concluded that post-translational processing of proglucagon differs in pancreas and intestine, so that the C-terminal portion of the molecule is cleaved to liberate free GLP-1 in the intestine. Further processing or degradation results in loss especially of GLP-2 immunoreactivity.

【 授权许可】

Unknown