FEBS Letters | |
Molecular forms of glucagon‐like peptides in man | |
Uttenthal, L.O.1  Bloom, S.R.1  George, S.K.1  Ghiglione, M.1  | |
[1] Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, London W12 OHS, England | |
关键词: Glucagon-like peptide; Proglucagon; Post-translational processing; Pancreas; Intestine; | |
DOI : 10.1016/0014-5793(85)80124-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Molecular forms of the glucagon-like peptides (GLP) encoded by the human preproglucagon gene were analysed by chromatography combined with specific radioimmunoassays to the synthetic peptides. Whereas extracts of human pancreas and a glucagonoma contained a large proglucagon cleavage product possessing both GLP-1 and GLP-2 immunoreactivities, extracts of human intestine contained products corresponding to free GLP-1 and a small amount ofchromatographically distinct GLP-2 immunoreactivity. It is concluded that post-translational processing of proglucagon differs in pancreas and intestine, so that the C-terminal portion of the molecule is cleaved to liberate free GLP-1 in the intestine. Further processing or degradation results in loss especially of GLP-2 immunoreactivity.
【 授权许可】
Unknown
【 预 览 】
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RO201912020287383ZK.pdf | 330KB | download |