Nucleotide sequence determination of chicken glucagon precursor cDNA

FEBS Letters

Takada, Tatsuyuki¹  Nata, Koji²  Yamamoto, Hiroshi²  Okamoto, Hiroshi²  Hasegawa, Shin¹  Terazono, Kimio² 

[1] Department of Animal Nutrition, Faculty of Agriculture, Kobe University, Kobe 657, Hyogo, Japan;Department of Biochemistry, Tohoku University School of Medicine, Sendai 980, Miyagi, Japan

关键词: Preproglucagon;    Glucagon;    Glucagon-like peptide;    mRNA;    cDNA cloning;    Gallus gallus domesticus;   

DOI  :  10.1016/0014-5793(90)80779-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151-amino acid long precursor, being preceded by a signal sequence and an amino-terminal peptide (NH₂-peptide) and followed by an intervening peptide and a glucagon-like peptide I (GLP-I). Chicken preproglucagon, however, lacked GLP-II and intervening peptide II which have been shown to be contained in mammalian glucagon precursors.

【 授权许可】

Unknown