Single‐turnover flash‐induced ATP synthesis in photosystem I‐enriched subchloroplast vesicles

FEBS Letters

Galmiche, J.M.¹  Kraayenhof, R.²  Girault, G.¹  De Wolf, F.A.¹ 

[1] Departement de Biologie, Service de Biophysique, Centre d Études Nucléaires de Saclay, 91191 Gif-sur-Yvette, France;Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

关键词: ATP synthesis;    Single turnover;    Photosystem I;    Subchloroplast vesicle;    (Spinach);    Ferredoxin Redox balance;    Chl;    chlorophyll;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-p-benzoqumone;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    DNP-INT;    2-iodo-6-isopropyl-3-methyl-2 ';    4;    4 '-triiritrodiphenyl ether;    FCCP;    carbonyl cyanide P-trifluoromethoxyphenyl-hydrazone;    HQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    PMS;    N -methylphenazonium methosulphate;    PS;    photosystem;   

DOI  :  10.1016/0014-5793(85)80123-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flash-induced ATP synthesis coupled to cyclic electron flow in photosystem I-enriched subchloroplast vesicles was studied by continuous registration of luciferin-luciferase luminescence. Cyclic electron flow was elicited in the presence of the artificial mediator phenazine methosulphate or of native ferredoxin, the latter redox-poised by NADPH and oxygen. ATP hydrolysis is observed in dark-adapted vesicles in the presence of ferredoxin without requirement for previous activation of the ATPases. Ferredoxin-catalyzed ATP synthesis proceeds at a constant (maximal) rate between NADPH/O₂; ratios of 1.5 and 11. It was sensitive to FCCP, DCCD, DBMIB, DNP-INT and HQNO, but not to antimycin A.

【 授权许可】

Unknown